Decontamination of Salmonella, Shigella, and Escherichia coli O157:H7 from Leafy Green Vegetables Using Edible Plant Extracts
Identifieur interne : 001077 ( Main/Exploration ); précédent : 001076; suivant : 001078Decontamination of Salmonella, Shigella, and Escherichia coli O157:H7 from Leafy Green Vegetables Using Edible Plant Extracts
Auteurs : Nydia Orue [Mexique] ; Santos García [Mexique] ; Peter Feng [États-Unis] ; Norma Heredia [Mexique]Source :
- Journal of Food Science [ 0022-1147 ] ; 2013-02.
Abstract
Fresh cilantro, parsley, and spinach are products that are regularly consumed fresh, but are difficult to decontaminate, as a result, they are common vehicles of transmission of enteropathogenic bacteria. In this study, the efficacy of plant extracts as alternatives for disinfection of cilantro, parsley, and spinach that were artificially contaminated with Salmonella, Escherichia coli O157:H7, and Shigella sonnei was determined. Edible plant extracts obtained using ethanol as the extraction solvent were tested to determine the minimum bactericidal concentration (MBC) and those that exhibited the lowest MBC were selected for further studies. Leaves of fresh greens were washed with sterile water and dried. For seeding, leaves were submerged in suspensions of 2 different concentrations of bacteria (1.5 × 108 and 1 × 105), dried, and then stored at 4 °C until use. To determine the effects of the extracts, inoculated leafy greens were submerged in a container and subjected to treatments with chlorine, Citrol®, or selected plant extracts. Each treatment type was stored at 4 °C for 0, 1, 5, and 7 d, and the bacterial counts were determined. From the 41 plant extracts tested, the extracts from oregano leaves and from the peel and pulp of limes were found to be as effective as chlorine or Citrol® in reducing by > 2 logs, the population of pathogenic bacteria on leafy greens and therefore, may be a natural and edible alternative to chemicals to reduce the risk of Salmonella, E. coli O157:H7 and S. sonnei contamination on leafy vegetables.
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DOI: 10.1111/1750-3841.12016
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In this study, the efficacy of plant extracts as alternatives for disinfection of cilantro, parsley, and spinach that were artificially contaminated with Salmonella, Escherichia coli O157:H7, and Shigella sonnei was determined. Edible plant extracts obtained using ethanol as the extraction solvent were tested to determine the minimum bactericidal concentration (MBC) and those that exhibited the lowest MBC were selected for further studies. Leaves of fresh greens were washed with sterile water and dried. For seeding, leaves were submerged in suspensions of 2 different concentrations of bacteria (1.5 × 108 and 1 × 105), dried, and then stored at 4 °C until use. To determine the effects of the extracts, inoculated leafy greens were submerged in a container and subjected to treatments with chlorine, Citrol®, or selected plant extracts. Each treatment type was stored at 4 °C for 0, 1, 5, and 7 d, and the bacterial counts were determined. From the 41 plant extracts tested, the extracts from oregano leaves and from the peel and pulp of limes were found to be as effective as chlorine or Citrol® in reducing by > 2 logs, the population of pathogenic bacteria on leafy greens and therefore, may be a natural and edible alternative to chemicals to reduce the risk of Salmonella, E. coli O157:H7 and S. sonnei contamination on leafy vegetables.</div></front></TEI><affiliations><list><country><li>Mexique</li><li>États-Unis</li></country><region><li>Maryland</li></region></list><tree><country name="Mexique"><noRegion><name sortKey="Orue, Nydia" sort="Orue, Nydia" uniqKey="Orue N" first="Nydia" last="Orue">Nydia Orue</name></noRegion><name sortKey="Garcia, Santos" sort="Garcia, Santos" uniqKey="Garcia S" first="Santos" last="García">Santos García</name><name sortKey="Heredia, Norma" sort="Heredia, Norma" uniqKey="Heredia N" first="Norma" last="Heredia">Norma Heredia</name></country><country name="États-Unis"><region name="Maryland"><name sortKey="Feng, Peter" sort="Feng, Peter" uniqKey="Feng P" first="Peter" last="Feng">Peter Feng</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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